DL-1,4-Dithiothreitol quick Details
Synonyms: Dithiothreitol; threo-1,4-Dimercapto-2,3-butanediol; Cleland’s reagent; DTT
CAS No.: 3483-12-3
Molecular Fomula: C4H10O2S2
Molecular weight: 154.25
Appearance: White free flowing powder,hygroscopic
||A White free flowing powder,hygroscopic
||Freely soluble in methanol, methylene chloride
||5%(w/v) Solution in water is clear and colorless
||Rf value of the sample is comparable with that of the standard.
||41°C ± 3°C
|Related SubstanceUV Spectroscopy
||Content of Oxidised DTT (Dihydroxydithiane) NMT 0.4%
|Assay (by Iodimetry)
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1. DL-1,4-Dithiothreitol is as a reducing agent and deprotecting agent for thiolated DNA. The thiolated DNA terminal sulfur atom tends to form a dimer in solution, especially in the presence of oxygen. This dimerization greatly reduces the efficiency of some coupling reaction experiments (such as the immobilization of DNA in biosensors); while adding DTT to the DNA solution and removing it after a period of reaction, the dimerization of DNA can be reduced.
2. DL-1,4-Dithiothreitolis also often used for the reduction of disulfide bonds in proteins and can be used to prevent intramolecular or intermolecular disulfide bonds of proteins formed between cysteines in proteins. However, DTT often cannot reduce the disulfide bonds embedded in the protein structure (solvent unreachable). The reduction of such disulfide bonds often requires denaturation of the protein (heating at high temperature or adding a denaturing agent such as 6M guanidine hydrochloride, 8M urea or 1% SDS). On the contrary, according to the difference in the rate of reduction of disulfide bonds in the presence of DTT, the degree of embedding can be judged.
DL-1,4-Dithiothreitol Packaging and Shipping
Packing: 25kg /drum
Delivery: By Express, by sea,by air.
The seal is stored in a cool, dry place.